Infertility of CD9-Deficient Mouse Eggs Is Reversed by Mouse CD9, Human CD9, or Mouse CD81; Polyadenylated mRNA Injection Developed for Molecular Analysis of Sperm–Egg Fusion
نویسندگان
چکیده
منابع مشابه
Tetraspanin CD9 regulates invasion during mouse embryo implantation.
The expression of tetraspanin CD9 was found on blastocysts in mice and endometrium epithelial cells in human and bovine. However, it remains unknown how CD9 is involved in the precise dialogue between embryo and uterus during early pregnancy. This study was designed to investigate the functional roles of CD9 in the embryo implantation with monoclonal antibody against CD9 protein (anti-CD9 mAb) ...
متن کاملMolecular cloning and characterization of chick CD9.
CD9 is a tetra-membrane-spanning glycoprotein involved in cell adhesion, migration, growth signaling and tumor cell metastasis. In this study a CD9 cDNA clone has been isolated from chick cDNA library. The chick CD9 clone contains an open reading frame of 224 amino acids. The deduced amino acid sequence from chick CD9 shows 63.3% identity with the human counterpart. Human and monkey CD9 upregul...
متن کاملHuman and mouse mast cells use the tetraspanin CD9 as an alternate interleukin-16 receptor.
Interleukin-16 (IL-16) induces the chemotaxis and activation of mast cells (MCs) and other cell types. While it has been concluded that CD4 is the primary IL-16 receptor on T cells, at least one other IL-16 receptor exists. We now show that the IL-16-responsive human MC line HMC-1 lacks CD4, and that the IL-16-mediated chemotactic and Ca2+ mobilization responses of this cell can be blocked by a...
متن کاملCD81 and CD9 work independently as extracellular components upon fusion of sperm and oocyte
When a sperm and oocyte unite into one cell upon fertilization, membranous fusion between the sperm and oocyte occurs. In mice, Izumo1 and a tetraspanin molecule CD9 are required for sperm-oocyte fusion as one of the oocyte factors, and another tetraspanin molecule CD81 is also thought to involve in this process. Since these two tetraspanins often form a complex upon cell-cell interaction, it i...
متن کاملResidues SFQ ( 173 - 175 ) in the large extracellular loop of CD 9 are required for gamete fusion
Sperm-egg binding and fusion initiate the development of a new organism, but the molecular mechanisms of gamete adhesion, gamete membrane fusion and associated signaling are still poorly understood. Recently, one egg surface protein, CD9, was shown to be essential for gamete fusion. The fertility of CD9-deficient female mice is severely reduced because membrane fusion ability is lost in CD9-def...
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ژورنال
عنوان ژورنال: Developmental Biology
سال: 2002
ISSN: 0012-1606
DOI: 10.1006/dbio.2002.0694